Dieter K. Schneider

Biology Department, 463
Brookhaven National Laboratory
Upton, NY 11973-5000

Dieter Schneider is a co-principal investigator in the Macromolecular Crystallography Research Resource (PXRR) which provides facilities and support at the National Synchrotron Light Source for the benefit of outside and in-house investigators.   The PXRR is supported by the NIH's National Center for Research Resources and the DOE Office of Biological and Environmental Research in its mission to create optimal facilities and environments for macromolecular structure determination by synchrotron X-ray diffraction.   With a staff of about 25, the PXRR innovates new access modes such as FedEx crystallography, builds new facilities, currently on the X29 undulator, advances automation, develops remote participation software, collaborates with outside groups, teaches novice users, and supports visting investigators with 7day, 20 hours staff coverage.

Research Interests:

Applications of neutron small-angle scattering for the structure determination of macromolecular complexes are the focus of this program. Development of scattering techniques, computational methods, and neutron instrumentation is pursued to maintain a vigorous outside user program on the H9B SANS Beamline at the High Flux Beam Reactor.

While the HFBR is shutdown, development of a neutron fiber diffraction camera is continued. A dual purpose instrument for the measurement of diffuse scattering and fibre diffraction was built jointly with the BNL Physics Department and is being tested at the National Institute of Standards and Technology. Neutron image plate detectors are used to record diffraction at a high resolution. The new capabilities will be applied by structural biologists who are deriving the organization of multicomponent macromolecular fibrous complexes, probing the conformational changes in contractile proteins, and determining intermolecular forces.

Currently I am working intensely on the organization of SAS99 which I will chair together with Sow-Hsin Chen. For details about the XIth International Conference on Small-Angle Scattering visit the conference website at http://sas99.bnl.gov/sas99.

Additionaly, I am the beamline scientist at the NSLS beam line X26C, which is operated by a four-membered PRT including the BNL Biology Department, Cold Spring Harbor Laboratory, the State University of New York at Stony Brook, and the Georgia Research Alliance.

Neutron fibre diffraction pattern of a crystalline TMV specimen in a capillary of 0.7 mm diameter recorded for 10 minutes at H9B.

Publications:

Skinner JM, Cowan M, Buono R, Nolan W, Bosshard H, Robinson HH, Heroux A, Soares AS, Schneider DK, and Sweet RM.
Integrated software for macromolecular crystallography synchrotron beamlines II: revision, robots and a database
Acta Cryst. D62(11), 1340-1347 (2006).   PubMed   Full Text
Shi W, Robinson H, Sullivan M, Abel D, Toomey J, Berman LE, Lynch D, Rosenbaum G, Rakowsky G, Rock L, Nolan B, Shea-McCarthy G, Schneider D, Johnson E, Sweet RM, and Chance MR.
Beamline X29: a novel undulator source for X-ray crystallography.
J Synch Rad 13, 365-372 (2006).   PubMed   Full Text
Robinson HH, Shi W, Sullivan M, Nolan W, Schneider DK, Berman L, Lynch D, Rock L, Rosenbaum G, Johnson E, Chance MR, and Sweet RM.
An undulator beamline for protein crystallography at the NSLS: Commissioning and operation of X29.
Synch Rad News 18(5), 27-31 (2005).   Full Text
Soares AS, Caspar DLD, Weckert E, Héroux A, Hölzer K, Schroer K, Zellner J, Schneider D, Nolan W, and Sweet RM.
Three beam interference is a sensitive measure of the efficacy of macromolecular refinement techniques.
Acta Cryst D59(10), 1716-1724 (2003). PubMed  Full Text
Dk Schneider, SH Chen, MS Capel, B Hsiao, G Kostorz, JS Pedersen, P Timmins, and GD Wignall.
Issue Preface: XIth International Conference on Small-Angle Scattering (BNL, May 17-20, 1999).
J Appl Cryst, 33(3), (2000).
DB Stone, PA Timmins, DK Schneider, I Krylova, CHI Ramos, FC Reinach and RA Mendelson.
The Effect of Regulatory Ca++ on the in situ Structures of Troponin C and Troponin I: A Neutron Scattering Study
J Mol Biol 281, 689-704 (1998) PubMed.
Imai M, Kato T, and Schneider D.
Fluctuations and growth of a cubic network observed in a nonionic surfactant system.
J Chem Phys 106, 9362-9371 (1997).
Mendelson RA, Schneider DK, and Stone DB.
Conformations of Myosin Subfragment 1 ATPase Intermediates from Neutron and X-ray Scattering.
J Mol Biol 256, 1-7 (1996). PubMed.
Wu C, Quesada MA, Schneider DK, Farinato R, Studier FW, and Chu B.
Polyacrylamide solutions for DNA sequencing by capillary electrophoresis: mesh sizes, separation and dispersion.
Electrophoresis 17, 1103-1109 (1996). PubMed.
Flanagan JM, Wall JS, Capel MS, Schneider DK, and Shanklin J.
Scanning transmission electron microscopy and small-angle scattering provide evidence that native Escherichia coli ClpP is a tetradecamer with an axial pore.
Biochemistry 34, 10910-10917 (1995). PubMed
Graziano V, Gerchman SE, Schneider DK, and Ramakrishnan V.
Histone H1 is located in the interior of the chromatin 30-nm filament.
Nature 368, 351-354 (1994). PubMed
Bivin DB, Stone DB, Schneider DK, and Mendelson RA.
Cross-helix separation of tropomyosin molecules in acto-tropomyosin as determined by neutron scattering.
Biophys J, 59, 880-888 (1991). PubMed
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