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Howard Robinson
Ph.D., Cell & Molecular Biology
University of Michigan, 1980
Biology Department, 463
tel: (631) 344-4789 |
Howard Robinson is a beam line scientist in the
Macromolecular Crystallography Research Resource (PXRR) which provides
facilities and support at the National Synchrotron Light Source for the benefit
of outside and in-house investigators. The PXRR is supported by the
NIH's National Center for Research Resources and the DOE Office of Biological
and Environmental Research in its mission to create optimal facilities and
environments for macromolecular structure determination by synchrotron X-ray
diffraction. With a staff of about 24, the PXRR innovates new access modes
such as FedEx crystallography, builds new facilities, currently on the X25
undulator, advances automation, develops remote participation software,
collaborates with outside groups, teaches novice users, and supports visting
investigators with 7day, 20 hours staff coverage.
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Research Interests:
High throughput x-ray crystallography: Improvements in data collection at synchrotron sites, and simplified analysis of structures determined from MAD data. Bio-structure engineering: Protein mechanical engineering, high temperature stabilization of proteins, swapping of catalytic assemblies into new proteins. Cryo-electron microscopy: Techniques for interpretation of low resolution structures with high resolution derived structural information. Howard Robinson's group focuses on facilitating access to synchrotrons for a broad spectrum of Biology and Biotechnology research scientists. Training is the primary tool used to provide this expanded access, and this training is a combination of experience at the beamline and also web-based training tutorials.
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Note: PDB files referenced below can be viewed with
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Kim S.K., Robinson H., Nelson B.C., Ready P.T. and Ladner J.E. Crystal Structure of Yersinia pestis Chorismate Mutase. To be Published |
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Han Q., Robinson H. and Li J. Crystal structure of human kynurenine aminotransferase II. J Biol Chem. 283(6):3567-3573. (2008) PubMed |
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Han Q.,Gao Y.G., Robinson H. and Li J. Structural insight into the mechanism of Substrate specificity of Aedes Kynurenine Aminotransferase II. Biochemistry. 47(6):1622-1630 (2008). PubMed |
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Buchko G.W., Robinson H., Pakrasi H.B. and Kennedy M.A. Insights into the structural variation between pentapeptide repeat proteins-Crystal structure of Rfr23 from Cyanothece 51142. J. Struct. Biol. epub Nov 28 (2007). PubMed |
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Fisher R.D., Chung H.Y., Zhai Q., Robinson H., Sundquist W.I. and Hill C.P. Structural and Biochemical Studies of ALIX/AIP1 and Its Role inRetrovirus Budding. Cell 128: 841-52 (2007). PubMed |
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Keiski C.L., Yip P., Robinson H., Burrows L.L. Howell P.L. Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas fluorescens AlgK. Acta Cryst. F63: 415-418 (2007). PubMed |
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Kelly B.N., Kyere S., Kinde I., Tang C., Howard B.R., Robinson H., Sundquist W.I., Summers M.F., Hill C.P. Structure of the Antiviral Assembly Inhibitor CAP-1 Complex with the HIV-1 CA Protein. J. Mol Biol. 373: 355-366 (2007). PubMed |
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Mans J., Natarajan K., Balbo A., Schuck P., Eikel D., Hess S., Robinson H., Simic H., Jonjic S., Tiemessen C.T and Margulies D.H. Cellular expression and crystal structure of the murine cytomegalovirus MHC-Iv glycoprotein, m153. J Biol Chem. 282: 35247-58 (2007). PubMed |
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Moore B.A., Robinson H.H. and Xu Z. The Crystal Structure of Mouse Exo70 Reveals Unique Features of the Mammalian Exocyst. J. Mol. Biol. 371: 1141-50 (2007). Medline Abstract. PubMed |
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Oruganti S., Zhang Y., Li H., Robinson H., Terns M.P., Terns R.M., Yang W., Li H. Alternative Conformations of the Archaeal Nop56/58-Fibrillarin Complex Imply Flexibility in Box C/D RNPs. J. Mol. Biol. 371: 1141-50 (2007). PubMed |
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Parson J.F., Greenhagen B.T., Shi K., Calabrese K., Robinson H. and Ladner J.E. Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa. Biochemistry 46: 1821-8 (2007). PubMed |
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Pfister T.D., Mirareli A.Y., Gengenbach A.J., Zhaoi X., Danstrom C., Conaster N., Gao Y.G., Robinson H., Zukoski C.F., Wang A.H. and Lu Y. Kinetic and crystallographic studies of a redesigned manganese-binding site in cytochrome c peroxidase. J. Biol. Inorg. Chem. 1: 126-37 (2007). PubMed |
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Wang H., Liu Y., Hou J., Zheng M., Robinson H. and Ke H. Structural insight into substrate specificity of phosphodiesterase 10. Proc Natl Acad Sci U S A. 104(14):5782-5787 (2007). PubMed |
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Wang H., Peng M., Chen Y., Geng J., Robinson H., Houslay M.D., Cai J. and Ke H. Structures of the four subfamilies of phosphodiesterase-4 provide insight into the selectivity of their inhibitors. Biochem J. 408: 193-201 (2007). PubMed |
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Wang H., Robinson H. and Ke H. The Molecular Basis for Different Recognition of Substrates by Phosphodiesterase Families 4 and 10. J. Mol. Biol. 371: 302-307 (2007). PubMed |
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Wang H., Ye M., Robinson H., Francis S.H. and Ke H. Conformational variations of both PDE5 and inhibitors provide the structural basis for the physiological effects of vardenafil and sildenafil. Mol Pharmacol. 73(1):104-110 (2007). PubMed |
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Zhang Y., Cao R., Leon A., Guo R.T., Krysiak K., Yin F., Hudock M.P., Mukherjee S., Gao Y.G., Robinson H., Song Y., No
J.H., Hong W., Morita C., Wang A.H.-J. and Oldfield E. Bisphosphonates: Teaching Old Drugs with New Tricks. |
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Alam S.L., Langelier C., Whitby F.G., Koirala S., Robinson H., Hill C.P. and Sundquist W.I. Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Nat Struct Mol Biol. 11: 1029-30 (2006). PubMed |
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Buchko G.W., Ni S., Robinson H., Welsh E.A., Pakrasi H.B. and Kennedy M.A. Characterization of two potentially universal turn motifs that shape the repeated five-residues fold--crystal structure of a lumenal pentapeptide repeat protein from Cyanothece 51142. Protein Sci. 11: 2579-95 (2006). PubMed |
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Buchko G.W., Ni S., Robinson H., Welsh E.A., Pakrasi H.B. and Kennedy M.A. Cloning, expression, crystallization and preliminary crystallographic analysis of a pentapeptide-repeat protein (Rfr23) from the bacterium Cyanothece 51142. Acta Cryst. F62: 1251-4 (2006). PubMed |
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Gallager D.T., Smith N.N., KIM S.K., Heroux A., Robinson H. and Reddy P.T. Structure of the Class IV Adenylyl Cyclase Reveals a Novel Fold. J Mol Biol. 362(1):114-122 (2006). PubMed |
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Han Q., Robinson H., Gao Y.G., Vogelaar N., Wilson S.R., Rizzi M. and Li J. Crystal structures of Aedes aegypti alanine glyoxylate aminotransferase. J Biol Chem. 281(48):37175-82 (2006). PubMed |
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Huai Q., Sun Y., Wang H., Macdonald D., Aspiotis R., Robinson H., Huang Z. and Ke H. Enantiomer Discrimination Illustrated by the High Resolution Crystal Structures of Type 4 Phosphodiesterase. J Med Chem. 49: 1867-1873 (2006). PubMed |
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Kelly N.B., Howard B.R., Wang H., Robinson H., Sundquist W.I. and Hill C.P. Implications for Viral Capsid Assembly from Crystal Structures of HIV-1 Gag(1)(-)(278) and CA(N)(133)(-)(278)(,). Biochemistry 45: 11257-66 (2006). PubMed |
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Natarajan K., Hicks A., Mans J., Robinson H., Guan R., Mariuzza R.A. and Margulies D.H. Crystal structure of the murine cytomegalovirus MHC-I homolog m144. J Mol Biol. 358(1): 157-71 (2006). PubMed |
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Ni S., Forouhar F., Bussiere D.E., Robinson H. and Kennedy M.A. Crystal structure of VC0702 at 2.0 A: conserved hypothetical protein from Vibrio cholerae. Proteins. 63: 733-41 (2006). PubMed |
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Robinson H., Soares A.S., Becker M., Sweet R. and Heroux A. Mail-in crystallography program at Brookhaven National Laboratory's National Synchrotron Light Source. Acta Cryst. D62(11): 1336-1339 (2006). PubMed Full Text |
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Sanghani P.C., Davis W.I., Zhai L. and Robinson H. Structure-function relationships in human glutathione-dependent formaldehyde dehydrogenase. Role of Glu-67 and Arg-368 in the catalytic mechanism. Biochemistry 18: 4819-30 (2006). PubMed |
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Shi W., Robinson H., Sullivan M., Abel D., Toomey J., Berman L.E., Lynch D., Rosenbaum G., Rakowsky G., Rock L., Nolan B.,
Shea-McCarthy G., Schneider D., Johnson E., Sweet R.M. and Chance M.R. Beamline X29: a novel undulator source for X-ray crystallography. J Synch Rad 13: 365-372 (2006). PubMed Full Text |
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Skinner J.M., Cowan M., Buono R., Nolan W., Bosshard H., Robinson H.H., Heroux A., Soares A.S., Schneider D.K. and Sweet R.M. Integrated software for macromolecular crystallography synchrotron beamlines II: revision, robots and a database Acta Cryst. D62(11): 1340-1347 (2006). PubMed Full Text |
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Wang H., Liu Y., Huai Q., Cai J., Zoraghi R., Francis S.H., Corbin J.D., Robinson H., Xin Z., Lin G. and Ke H. Multiple conformations of phosphodiesterase-5: implications for enzyme function and drug development. J Biol Chem. 281(30):21469-21479 (2006). PubMed |
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Bitto E., Bingman C.A., Robinson H., Allard S.T.M., Wesenberg G.E., and Phillips Jr G.N. The structure at 2.5 Å resolution of human basophilic leukemia-expressed protein BLES03. Acta Cryst Section F61(9): 812-817 (2005). PubMed |
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Brown J.H., Zhou Z., Reshetnikova L., Robinson H., Yammani R.D., Tobacman L.S. and Cohen C. Structure of the mid-region of tropomyosin: Bending and binding sites for actin. Proc Natl Acad Sci USA. 102(52): 18878-18883 (2005). PubMed Full Text PDB file 2B9C Jmol viewer |
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Forster A., Masters E.I., Whitby F.G., Robinson H. and Hill C.P. The 1.9 Å structure of proteasome-11S activator complex and implications for proteasome-PAN/PA700 interactions. Mol Cell 18: 589-599 (2005). PubMed PDB files 1YAR, 1Z7Q Jmol viewer |
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Han Q., Gao Y.G., Robinson H., Ding H., Wilson S. and Li J. Crystal structures of Aedes aegypti kynurenine aminotransferase. FEBS J. 272(9): 2198-2206 (2005). PubMed PDB files 1YIY, 1YIZ Jmol viewer |
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Li F., Robinson H., and Yeung E.S. Automated high-throughput nanoliter-scale protein crystallization screening. Anal Bioanal Chem, 383(7-8): 1034-1041 (2005). PubMed |
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Robinson H.H., Shi W., Sullivan M., Nolan W., Schneider D.K., Berman L., Lynch D., Rock L., Rosenbaum G., Johnson E., Chance M.R. and Sweet R.M. An undulator beamline for protein crystallography at the NSLS: Commissioning and operation of X29. Synch Rad News 18(5): 27-31 (2005). Full Text |
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Safo M.K., Zhao Q., Ko T.-P., Musayev F.N., Robinson H., Scarsdale N., Wang A.H.-J., and Archer G.L. Crystal structures of the BlaI repressor from Staphylococcus aureus and its complex with DNA: Insights into transcriptional regulation of the bla and mec operons. J Bacteriol. 187(5): 1833-1844 (2005). PubMed Full Text PDB files 1SD4, 1ZSD Jmol viewer |
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Wang H., Liu Y., Chen Y., Robinson H. and Ke H. Multiple elements jointly determine inhibitor selectivity of cyclic nucleotide phosphodiesterases 4 and 7. J Biol Chem 280(35): 30949-30955 (2005). PubMed Full Text PDB file 1ZKL Jmol viewer |
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Gallagher D.T., Monbouquette H.G., Schroder I., Robinson H., Holden M.J. and Smith N.N. Structure of alanine dehydrogenase from Archaeoglobus: Active site analysis and relation to bacterial cyclodeaminases and mammalian mu crystallin. J Mol Biol. 342(1): 119-130 (2004). PubMed PDB file 1OMO Jmol viewer |
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Hou M.H., Robinson H., Gao Y.G. and Wang A.H.J. Crystal structure of the [Mg2+-(chromomycin A3)2]-d(TTGGCCAA)2 complex reveals GGCC binding specificity of the drug dimer chelated by a metal ion. Nucl Acids Res. 32(7): 2214-2222 (2004). PubMed Full Text PDB file 1VAQ Jmol viewer |
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Huai Q., Wang H., Zhang W., Colman R.W., Robinson H. and Ke H. Crystal structure of phosphodiesterase 9 shows orientation variation of inhibitor 3-isobutyl-1-methylxanthine binding. Proc Natl Acad Sci USA 101(26): 9624-9629 (2004). PubMed Full Text PDB file 1TBM Jmol viewer |
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Karthikeyan S., Zhou Q., Zhao Z., Kao C.L., Tao Z., Robinson H., Liu H.W. and Zhang H. Structural analysis of Pseudomonas 1-aminocyclopropane-1-carboxylate deaminase complexes: Insight into the mechanism of a unique pyridoxal-5'-phosphate dependent cyclopropane ring-opening reaction. Biochemistry 43(42): 13328-13339 (2004). PubMed PDB files 1TZ2, 1TZK, 1TZM, 1TYZ, 1TZJ Jmol viewer |
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Mao J., Gao Y.G., Odeh S., Robinson H., Montalvetti A., Docampo R. and Oldfield E. Crystallization and preliminary X-ray diffraction study of the farnesyl diphosphate synthase from Trypanosoma brucei. Acta Cryst D60(10): 1863-1866 (2004). PubMed |
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Ni S., Robinson H., Marsing G.C., Bussiere D.E. and Kennedy M.A. Structure of 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase from Shewanella oneidensis at 1.6 Å: Identification of farnesyl pyrophosphate trapped in a hydrophobic cavity. Acta Cryst Section D60: 1949-1957 (2004). PubMed PDB file 1T0A Jmol viewer |
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Phillips J.D., Whitby F.G., Warby C.A., Labbe P., Yang C., Pflugrath J.W., Ferrara J.D., Robinson H., Kushner J.P. and Hill C.P. Crystal structure of the oxygen-dependant coproporphyrinogen oxidase (Hem13p) of Saccharomyces cerevisiae. J Biol Chem. 279(37): 38960-38968 (2004). PubMed Full Text PDB files 1TK1, 1TKB, 1TKL Jmol viewer |
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Saxena S., Yuan P., Dhar S.K., Senga T., Takeda D., Robinson H., Kornbluth S., Swaminathan K. and Dutta A. A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition. Mol Cell 15(2): 245-258 (2004). PubMed PDB file 1UII Jmol viewer |
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Schwarzenbacher R., Stenner-Liewen F., Liewen H., Robinson H., Yuan H., Bossy-Wetzel E., Reed J.C. and Liddington R.C. Structure of the Chlamydia protein CADD reveals a redox-enzyme that modulates host cell apoptosis. J Biol Chem 279(28): 29320-29324 (2004). PubMed Full Text PDB file 1RCW Jmol viewer |
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Smith N., Mayhew M., Holden M.J., Kelly H., Robinson H., Héroux A., Vilker V.L. and Gallagher D.T. Structure of C73G putidaredoxin from Pseudomonas putida. Acta Cryst D60(5): 816-822 (2004). PubMed PDB file 1R7S Jmol viewer |
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Woo E.J., Kim Y.G., Kim M.S., Han W.D., Shin S., Robinson H., Park S.-Y. and Oh B.-H. Structural mechanism for inactivation and activation of CAD/DFF40 in the apoptotic pathway. Mol Cell 14(4): 531-539 (2004). PubMed PDB file 1V0D Jmol viewer |
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Fisher R.D., Wang B., Alam S.L., Higginson D., Rich R., Myszka D., Robinson H., Sundquist W.I. and Hill C.P. Structure and ubiquitin binding of the ubiquitin interacting motif. J. Biol. Chem. 278 (31): 28976-84 (2003). PubMed Full Text PDB file 1O06 |
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Huai Q., Sun Y., Wang H., Chin L.S., Li L., Robinson H. and Ke H. Crystal structure of DJ-1/RS and implication on familial Parkinson's disease. FEBS Lett. 549(1-3): 171-175 (2003). PubMed PDB files 1PS4, 1Q2U |
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Ko T.P., Robinson H., Gao Y.G., Chen C.H., DeVries A.L. and Wang A.H.J. The refined crystal structure of an eel pout type III antifreeze protein RD1 at 0.62-A resolution reveals structural micorheterogeneity of protein and solvation. Biophys. J. 84(2): 1228-1237 (2003). PubMed PDB file 1UCS |
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Paaventhan P., Joseph J.S., Seow S.V., Vaday S., Robinson H., Chua K.Y. and Kolatkar P.R. A 1.7A structure of Fve, a member of the new fungal immunomondulatory protein family. J Mol Biol. 332(2): 461-470 (2003). PubMed PDB file 1OSY |
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Sanghani P.C., Robinson H., Bennett-Lovsey R., Hurley T.D. and Bosron W.F. Structure-function relationships in human Class III alcohol dehydrogenase (formaldehyde dehydrogenase). Chem. Biol. Interact. 143-144: 195-200 (2003). PubMed |
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Smith N., Mayhew M., Robinson H., Héroux A., Charlton D., Holden M.J. and Gallagher D.T. Crystallization and phasing of alanine dehydrogenase from Archaeoglobus fulgidus. Acta Cryst. D59(12): 2328-2331 (2003). PubMed |
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Singh S.K., Kurnasov O.V., Chen B., Robinson H., Grishin N.V., Osterman A.L. and Zhang H. Crystal structure of Haemophilis influenzae NadR protein. A bifunctional enzmye endowed with NMN adenyltransferase and ribosylnicotinimide kinase activities. J Biol Chem. 277(36): 33291-33299 (2002). PubMed Full Text PDB file 1LW7 |
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Sanghani P.C., Robinson H., Bosron W.F. and Hurley T.D. Human glutathione-dependent formaldehyde dehydorgenase. Structural basis of the random kinetic mechanism. Biochemistry 41(35): 10778-10786 (2002). PubMed PDB files 1M6H, 1M6W, 1MAO |
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Hou M.-H., Robinson H., Gao J.G. and Wang A.H.J. Crystal structure of Actinomycin D bound to the CTG triplet repeat sequences linked to neurological diseases. Nucleic Acids Res. 30(22): 4910-4917 (2002). PubMed PDB file 1MNV |
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Ko T.P., Chen Y.K., Robinson H., Tsai P.C., Gao Y.G., Chen A.P., Wang A.H. and Liang P.H. Mechanism of product chain length determination and the role of flexible loop in Escherichia coli undecaprenyl pyrophosphate synthase J Biol Chem. 276(50): 47474-82 (2001). PubMed Full Text PDB file 1JP3 |
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Robinson H., Gao Y.G., Yang X.L., Sanishvili R., Joachimiak A. and Wang A.H. Crystallographic analysis of a novel complex of actinomycin D bound to the DNA decamer CGATCGATCG. Biochemistry 40(19): 5587-5592 (2001). PubMed PDB file1I3W |
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Robinson H., Gao Y.G., Sanishvili R., Joachimiak A., and Wang A.H.J. Hexaydrated magnesium ions bind to the deep major groove and at the outer mouth of A-form nucleic acid duplexes. Nucleic Acids Res. 28: 1760-1766 (2000). PubMed PDB files 1DNZ, 1DNO, 1DNT, 1DNX |
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Su S., Gao Y.G., Robinson H., Liaw Y.C., Edmondson S.P., Shriver J.W. and Wang A.H. Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base pairs. J Mol Biol. 303(3): 395-404 (2000). PubMed PDB files 1C8C, 1CA5, 1CA6 |
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Yang X.L., Robinson H., Gao Y.G., and Wang A.H.J. Binding of a macrocyclic bisacridine and amentantrone to CGTACG involves similar unusual intercalation platforms. Biochemistry 39(36): 10950-10957 (2000). PubMed PDB files 1FDG, 1FD5 |
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Robinson H., Ang M.C., Gao Y.G., Hay M.T., Lu Y. and Wang A.H.J. Structural basis of electron transfer modulation in the purple Cu-A center. Biochemistry 38: 5677-5683 (1999). PubMed PDB file 1CC3 |
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Robinson H., Gao Y.G., McCrary B.S., Edmondson S.P., Shriver J.W. and Wang A.H.J. The hyperthermophile chromosomal protein Sac7d sharply kinks DNA. Nature 392: 202-205 (1998). PubMed PDB files 1AZQ, 1AZP, 1CA5, 1CA6 |
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Robinson H., Priebe W., Chaires J.B., and Wang A.H.J. Binding of two novel bisdaunorubicins to DNA studied by NMR spectroscopy. Biochemistry 36: 8663-8670 (1997). PubMed PDB files 1AL9, 1AMD |
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