John C. Sutherland

Biology Department, 463
Brookhaven National Laboratory
Upton, NY 11973-5000

Research Interests:

John Sutherland uses optical and other physical methods to study the structure, function and dynamics of DNA, proteins and other biological materials. He developed theory and electronic imaging systems for quantifying DNA damage by gel electrophoresis and the method-of-moments, and participated in studies of the damage induced by UV in DNA in human skin and plants in situ, the repair of this damage, and the environmental implications of depletion of stratospheric ozone. Similar approaches are being used to quantify DNA damage induced by ionizing radiations. He received an R&D-100 award for invention of the first system for quantitative imaging of fluorescence from fluorophore labeled DNA in electrophoretic gels with a CCD camera.

He pioneered the use of synchrotron radiation for the measurement of circular dichroism and time-resolved fluorescence spectroscopy in the ultraviolet/visible spectral regions. He received a R&D-100 award for invention of the Fluorescence Omnylizer, a single-photon counting detector that simultaneously records the time-delay, wavelength and polarization of each detected photon. He also participated in the design and construction of beamline X13A at the NSLS that measures circular dichroism of soft X-ray using phase-sensitive detection. Current interests include free-electron lasers and other next generation accelerator-based light sources as probes of biomolecular structure and dynamics. He manages NSLS beamlines U9B and U11 and their attendant external user program for far-ultraviolet circular dichroism and time-resolved fluorescence spectroscopy.

Selected References:
Miles A.J., Janes R.W., Brown A., Clarke D.T., Sutherland J.C., Tao Y., Wallace B.A. and Hoffmann S.V.
Light Intensity Threshold at Which Protein Denaturation is Induced by Synchrotron Radiation Circular Dichroism (SRCD) Beamlines.
J Synchrotron Radiation. (submitted).
Sutherland J. C. and Wallace B. A.
BioCD-2007 Workshop.
Synchrotron Radiation News, (in press).
Sutherland B.M., Bennett P.V. and Sutherland J.C.
DNA damage quantitation by alkaline gel electrophoresis.
Methods Mol Biol, 314: 251-73 (2006). PubMed
Sutherland J.C.
Repair dependent radiation survival: a stochastic model with Euler gamma function solutions.
Physics in Medicine and Biology, 51: 4883-4901 (2006). PubMed
Sutherland J.C.
BioCD-2005 Workshop.
Synchrotron Radiation News, 18(6): 29 (2005).
Filippova E.M., Monteleone D.C., Trunk J.G., Sutherland B.M., Quake S.R. and Sutherland J.C.
Quantifying Damage to Double Stranded DNA by Single Molecule Laser Fluorescence Sizing.
Biophysical, 84: 1281-1290 (2003).
Praissman J.L. and Sutherland J.C.
Laboratory Voice Data Entry System, in BioComputing: computer tools for Biologists.
S.M. Brown, Editor. Eaton Publishing: Westborough, MA., 493-502 (2003).
Sutherland B.M., Georgakilas A.G., Bennett P.V., Laval J. and Sutherland J.C.
Quantifying Clustered DNA Damage Induction and Repair by Gel Electrophoresis, Electronic Imaging and Number Average Length Analysis.
Mutation Research, 531(1-2): 93-107 (2003). PubMed
Sutherland B.M., Bennett P.V., Georgakilas A.G. and Sutherland J.C.
Evaluation of Number Average length Analysis in Quantifying Double Strand Breaks in Genomic DNAs.
Biochemistry, 42(11): 3375-3384 (2003). PubMed
Sutherland B.M., Bennett P.V., Sutherland J.C. and Laval J.
Clustered DNA damages induced by X-rays in human cells.
Radiation Research, 156: 611-616 (2002). PubMed
Sutherland B.M., Hacham H., Bennett P., Sutherland J.C., Moran M. and Gange R.W.
Repair of cyclobutyl pyrimidine dimers in human skin: variability among normal humans in nucleotide excision and in photorepair.
Photodermatol Photoimmunol Photomed, 18: 109-116 (2002). PubMed
Sutherland B.M., Cintron-Torres N., Bennett P.V., Hada M., Schenk H., Trunk J.G., Monteleone D.C., Sutherland J.C., Laval J., Stanislaus M. and Gewirtz A.
Clustered DNA damages induced in human hematopoietic cells by low doses of ionizing radiation.
Journal of Radiation Research, 43: S149-S152 (2002). PubMed
Sutherland J.C.
2002 X-ray Sources for Studies of Ultrafast Processes.
Synchrotron Radiation News, 15(5): 12-13 (2002).
Sutherland J.C.
Biological Effects of Polychromatic Light.
Photochemistry and Photobiology, 76: 164-170 (2002). PubMed
Sutherland J.C.
Simultaneous measurement of circular dichroism and fluorescence polarization anisotropy.
In: Cohn GE, editor. Proceedings of SPIE, 4625: 126-136 (2002).
Bennett P.V., Hada M., Hidema J., Lepre A.M., Pope L.C., Quaite F.E., Sullivan J.H., Takayanagi S., Sutherland J.C. and Sutherland B.M.
Isolation of High Molecular Length DNA: Alfalfa, Pea, Rice, Sorghum, Soybean and Spinach Crop.
Science, 167-172 (2001).
Sutherland B.M., Bennett P.V., Saparbaev M., Sutherland J.C. and Laval J.
Clustered DNA damages as dosemeters for exposure and biological responses.
Radiation Protection Dosimetry, 97: 33-38 (2001). PubMed
Sutherland B.M., Bennett P.V., Schenk H., Sidorkina O., Laval J., Trunk J., Monteleone D., and Sutherland J.C.
Clustered DNA damages induced by high and low LET radiation, including heavy ions.
Physica Medica, 17S1: 204-206 (2001).
Sutherland J.C., Monteleone D.C. and Trunk J.G.
The Integrating Ion Imager: A Device for Determining Heavy Ion Doses During Irradiations.
Physica Medica, 17S1: 139-142 (2001). PubMed
Sutherland J.C., Monteleone D.C., Trunk J.G., Bennett P.V. and Sutherland B.M.
Quantifying DNA Damage by Gel Electrophoresis, Electronic Imaging and Number-Average Length Analysis.
Electrophoresis, 22: 843-854 (2001). PubMed
Dylla H.F., Benson S.V., Neil G.R., Shinn M., Austin R.H. and Sutherland J.C.
Applications of the Jefferson Lab free electron laser for photobiology.
Proceedings of the Society of Photo-Instrumentation Engineers, 3925: 40-49 (2000).
Johnson E.D., Ben-Zvi I., DiMauro L.F., Graves R.N., Heese R.M., Krinsky S., Sutherland J.C., Wang X.J. and Yu L.
Deep ultraviolet free electron laser (DUV-FEL) at Brookhaven National Laboratory.
Proceedings of the Society of Photo-Instrumentation Engineers, 3925: 26-29 (2000).
Oakberg T.C., Trunk J. and Sutherland J.C.
Calibration of photoelastic modulators in the vacuum UV.
Proceedings of the Society for Photo-Optical Instrumentation Engineers, 4133: 101-111 (2000).
Sutherland J.C.
Can Free Electron Lasers Answer Critical Questions in Ultraviolet Photobiology?
Proceedings of the Society of Photo-Instrumentation Engineers, 3925: 50-59 (2000).
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