S. Eswaramoorthy Biology Department, 463 Brookhaven National Laboratory Upton, NY 11973-5000 tel: (631) 344-7402 fax: (631) 344-3407 eswar@bnl.gov

S. Eswaramoorthy is a scientist in the Structural Genomics Group at the BNL Biology Department which is a partner in the New York Structural Genomics Research Consortium. The NYSGRC is a collaboratory of the Albert Einstein College of Medicine, BNL, Columbia University, Structural Genomics Inc., The Rockefeller University, University of California at San Francisco, and the Sloan Kettering Institute. The BNL Biology group is advancing the efficient crystallographic structure determination of NYSGRC targeted proteins, and currently includes the following scientists and post doctoral fellows: S. Swaminathan (Principal Investigator), S. Eswaramoorthy, D. Kumaran, R. Agarwal, S. Jayaraman, N. Krishnamurthy, and J-S. Jiang.

Research Interests: Our interests are to understand the function of selected proteins such as toxins and amino-acid processing enzymes through crystallographic structure analysis. The plant toxin ricin has been known for decades and its catalytic activity is studied well. Generally, the functional mechanism of toxins involves cell binding, internalization, and catalytic activity. Internalization or cell permeation is the part that needs more understanding and will yield many useful results. Our effort is to study the cell binding and internalization of ricin toxin through crystal structure analysis of ricin B chain - peptide/oligosaccharide complexes. The peptides that have high binding affinity to the toxin are selected from phage display libraries. The results from study are a prerequisite for toxin detection and vaccine development. Glutamine is one of the most abundant amino acids in the body and is found in all tissues. The metabolism of glutamine requires the enzyme, glutaminase. Glutaminase hydrolyzes the amide nitrogen of glutamine and produces glutamate and an ammonium ion. We are studying by crystallographic methods the structure function relationships of glutaminase.

Publications:

Note: Selected PDB files below can be viewed with

Madegowda M., Eswaramoorthy S., Burley S.K. and Swaminathan S. X-ray crystal structure of the B component of Hemolysin BL from Bacillus cereus. Proteins, (2008) [Epub ahead of print]. PubMed

Almo S.C., Bonanno J.B., Sauder J.M., Emtage S., Dilorenzo T.P., Malashkevich V., Wasserman S.R., Swaminathan S., Eswaramoorthy S., Agarwal R., Kumaran D., Madegowda M., Ragumani S., Patskovsky Y., Alvarado J., Ramagopal U.A., Faber-Barata J., Chance M.R., Sali A., Fiser A., Zhang Z.Y., Lawrence D.S. and Burley S. K. Structural genomics of protein phosphatases. Journal of Structural and Functional Genomics, 8(2-3): 121-140 (2007). PubMed

Eswaramoorthy S., Bonanno J.B., Burley S.K. and Swaminathan S. Mechanism of action of a flavin-containing monooxygenase. Proceedings of the National Academy of Sciences USA, 103(26): 9832-9837 (2006). PubMed

Jayaraman S., Eswaramoorthy S., Ahmed S.A., Smith L.A. and Swaminathan S. N-terminal helix reorients in recombinant C-fragment of Clostridium botulinum type B. Biochem Biophys Res Commun., 330(1): 97-103 (2005).  PubMed

Jayaraman S., Eswaramoorthy S., Kumaran D. and Swaminathan S. Common binding site for disialyllactose and tri-peptide in C-fragment of tetanus neurotoxin. PROTEINS: Structure, Function, and Bioinformatics, 61(2): 288-295 (2005). PubMed

Kumaran D., Eswaramoorthy S., Studier F.W. and Swaminathan S. Structure and mechanism of ADP-ribose-1''-monophosphatase (Appr-1''-pase), a ubiquitous cellular processing enzyme. Protein Sci. 14(3): 719-726 (2005).  PubMed

Agarwal R., Eswaramoorthy S., Kumaran D., Dunn J.J. and Swaminathan S. Cloning, high level expression, purification, and crystallization of the full length Clostridium botulinum neurotoxin type E light chain. Protein Expr Purif. 34(1): 95-102 (2004). PubMed

	Agarwal R., Eswaramoorthy S., Kumaran D., Binz T. and Swaminathan S. Structural analysis of botulinum neurotoxin type E catalytic domain and its mutant Glu212-->Gln reveals the pivotal role of the Glu212 carboxylate in the catalytic pathway. Biochemistry, 43(21): 6637-6644 (2004).  PubMed PDB files: 1T3A, 1T3C   Jmol viewer
	Eswaramoorthy S., Kumaran D., Keller J. and Swaminathan S. Role of Metals in the biological activity of Clostridium botulinum neurotoxins. Biochemistry, 43(8): 2209-2216 (2004).  PubMed PDB files: 1S0B, 1S0C, 1S0D, 1S0E, 1S0F, 1S0G    Jmol viewer
	Eswaramoorthy S., Rao S.T., Pan B. and Sundaralingam M. Structure of the dodecamer r(GAUCACUUCGGU) with four 5'-overhang nucleotides. Acta Cryst., D60(1): 8-12 (2004).  PubMed

Swaminathan S., Eswaramoorthy S. and Kumaran D. Structure and enzymatic activity of botulinum neurotoxins. Mov. Disord. Suppl., 8: S17-22 (2004). PubMed

	Eswaramoorthy S., Gerchman S., Graziano V., Kycia H., Studier F.W. and Swaminathan S. Structure of a yeast hypothetical protein selected by a structural genomics approach. Acta Cryst. D59(1): 127-135 (2003). PubMed PDB files: 1B54, 1CT5  Jmol viewer Click on icon to enlarge cover.
	Kumaran D., Eswaramoorthy S., Gerchman S.E., Kycia H., Studier F.W. and Swaminathan S. Crystal structure of a putative CN hydrolase from yeast. Proteins, 52(2): 283-291 (2003). PubMed PDB file: 1F89   Jmol viewer
	Eswaramoorthy S., Kumaran D. and Swaminathan S. A novel mechanism for Clostridium botulinum neurotoxin inhibition. Biochemistry, 41(31): 9795-9802 (2002). PubMed PDB files: 1G9A, 1G9B, 1G9C, 1G9D   Jmol viewer
	Eswaramoorthy S., Kumaran D. and Swaminathan S. Crystallographic evidence for doxorubicin binding to the receptor-binding site in Clostridium botulinum neurotoxin B. Acta Cryst., D57(11): 1743-1746 (2001).  PubMed PDB file: 1I1E  Jmol viewer
	Kumaran D., Eswaramoorthy S., Furey W., Sax M. and Swaminathan S. Structure of staphylococcal enterotoxin C2 at various pH levels. Acta Cryst., D57(9): 1270-1275 (2001).  PubMed PDB files: 1CQV, 1I4P, 1I4Q, 1I4R, 1I4X   Jmol viewer
	Kumaran D., Eswaramoorthy S., Luft B.J., Koide S., Dunn J.J., Lawson C.L. and Swaminathan S. Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi. EMBO J., 20(5): 971-978 (2001).  PubMed   Full Text (pdf) PDB files: 1F1M, 1GGQ  Jmol viewer
	Kumar P.R., Eswaramoorthy S., Vithayathil P.J.and Viswamitra M.A. The tertiary structure at 1.59 A resolution and the proposed amino acid sequence of a family-11 xylanase from the thermophilic fungus Paecilomyces varioti bainier. J. Mol. Biol., 295(3): 581-593 (2000).  PubMed PDB file: 1PVX  Jmol viewer
	Swaminathan S. and Eswaramoorthy S. Structural analysis of the catalytic and binding sites of Clostridium botulinum neurotoxin B. Nat. Struct. Biol., 7(8): 693-699 (2000).  PubMed PDB files: 1EPW, 1F31   Jmol viewer
	Sekar K., Eswaramoorthy S., Jain M.K. and Sundaralingam M. Crystal structure of the complex of bovine pancreatic phospholipase A2 with the inhibitor 1-hexadecyl-3-(trifluoroethyl)-sn-glycero-2-phosphomethanol. Biochemistry, 36(46): 14186-14191 (1997).  PubMed PDB file: 1FDK  Jmol viewer

Eswaramoorthy S., Vithayathil P.J. and Viswamitra M.A. Crystallization and preliminary X-ray crystallographic studies of thermostable xylanase crystals isolated from Paecilomyces varioti. J. Mol. Biol., 243(4): 806-808 (1994).  PubMed

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