Filament
and
Trace
Profile
|
-
Read in an image, run background, select a model, and position the cursor near the
center of the filament of interest.
-
After the model 'locks on' (difference image shows only noise), strike the <7>
key. The program should trace the centerline to one end of the filament, then return to the
starting point and trace to the other end. During the tracing, a plot should appear in the
bottom left on 1024x1280 displays and middle right on 768x1024 displays. The curves are:
local radius of curvature or twist angle (blue) relative to offset zero (gray),
local RMS difference between model and LA data (white/red),
local peak signal (green) and local M/L (yellow).
The x-coordinate is the distance from the end of the filament. Note that the plot reverses
when the search returns to the starting point and goes in the other direction.
-
After the first pass is complete, a second pass averages the radial profiles for segments
passing RMS and BKG_RMS tests. A final pass re-plots the traces with solid lines.
The 'Total Length' printed is the contour length of trace (both white & red in RMS plot).
-
Inspect the plot by holding down the <SPACE> bar and moving the mouse pointer
over the plot area. Cursors should indicate the position being read and the value at that point.
Note that for long filaments on a small screen (1024x768) the rightmost portion of the trace may
be cut off. The left and right arrow keys also move along the trace.
-
Save the data by holding down <CONTROL> and striking <7>. This writes
a file NKXXXXYYRadPro_xxx_yyy.txt in the MassMeas directory. The values xxx and yyy are coordinates
of one end of the filament. The average radial profile appears first, followed by the data as a
function of distance from the end. The 'radius of curvature' plot is actually 1000/Radius in pixels,
in order to have straight filaments = 0.
-
Save the entire screen to the clipboard by holding down <ALT> while hitting PrintScreen,
then paste into Paint, PowerPoint, or Photoshop and clip off unwanted information or save as a jpg file.
|
Tutorials 1
2
3
4
5
6
7
