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Biology Department Brookhaven National Laboratory |
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Membrane Diffractometer A User Facility at the HFBR |
H3B |
Contact:
Anand M. Saxena
Biology Department, 463 Brookhaven National Laboratory Upton, NY 11973-5000 |
tel: (516) 344-4844 fax: (516) 344-3407 asaxena@bnl.gov |
The H3B Group: H3B Beamline (516) 344-2278 John Lara (516) 344-4440 |
jlara@bnl.gov |
The H3B Membrane Neutron SpectrometerThe H3B spectrometer is designed for the study of partially ordered systems, such as stacked biological membranes and model membranes. A typical sample consists of a lipid-water multilayer, which may contain reconstituted integral membrane proteins, drugs, cholesterol, or other molecules whose location inside the membrane is to be determined. Selective deuteration of the membrane components and H2O-D2O exchange is applied in elucidating the structure. The spectrometer and associated equipment are controlled by a VAX 4000-400, and various types of sample holders are available. Software packages have been developed to assist the users in data analyses and the determination of scattering density profiles accross membranes. |
| Beamline Parameters | |
| Wavelength Range | 2 - 4 Å |
| Wavelength Bandwidth | 3 - 8 % |
| Beam Dimensions | 1 x 2 cm² (H x V) |
| Detector Area | 18 x 18 cm² |
| Detector Sampling | 192 x 128 pixel² (H x V) |
| Detector Resolution | H: 1.3 mm; V: 2.5 mm |
| Sample-to-Detector Distance | 0.5 - 2.5 m |
| Angular Scattering Range | -5° - 40° |
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