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Annie Héroux
Biology Department, 463
tel: (631) 344-4454
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Annie Héroux is a beam line scientist in the
Macromolecular Crystallography Research Resource (PXRR) which provides
facilities and support at the National Synchrotron Light Source for the benefit
of outside and in-house investigators. The PXRR is supported by the
NIH's National Center for Research Resources and the DOE Office of Biological
and Environmental Research in its mission to create optimal facilities and
environments for macromolecular structure determination by synchrotron X-ray
diffraction. With a staff of about 24, the PXRR innovates new access modes
such as FedEx crystallography, builds new facilities, currently on the X25
undulator, advances automation, develops remote participation software,
collaborates with outside groups, teaches novice users, and supports visting
investigators with 7day, 20 hours staff coverage.
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Research Interests and Institutional Responsibilities:
Beam Line Scientist at X25: As the beam line scientist at the macromolecular crystallography beam line X25, I oversee, test, and apply experimental techniques and structure determination methods that benefit the visiting scientists and the PXRR. A current focus is the shaping of the PXdb from the experimentor's point of view. The PXdb is our data base program that captures all aspects of data collection at the PXRR beamlines from project definition, to detailed journaling of data collections. Mail-in Crystallography:. In addition, I manage the PXRR Mail-in Crystallography Program. This team includes Howard Robinson and Alexei Soares, as well as our PX support staff in carrying out an extensive remote participation data collection effort. |
| Publications: |
Note: PDB files referenced below can be viewed with
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Hall B.M., Roberts S.A., Heroux A. and Cordes M.H. Two structures of a lambda Cro variant highlight dimer flexibility but disfavor major dimer distortions upon specific binding of cognate DNA. J Mol Biol. 375(3): 802-811 (2008). PubMed |
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Pallan P.S., Ittig D., Heroux A., Wawrzak Z., Leumann C.J. and Egli M. Crystal structure of tricyclo-DNA: an unusual compensatory change of two adjacent backbone torsion angles. Chemical Communications (Cambridge) 2008(7):883-885 (2008). PubMed |
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Petrie E.J., Clements C.S., Lin J., Sullivan L.C., Johnson D., Huyton T., Heroux A., Hoare H.L., Beddoe T., Reid H.H., Wilce M.C.J.,
Brooks A.G. and Rossjohn J. CD94-NKG2A recognition of human leukocyte antigen (HLA)-E bound to an HLA class I leader sequence. Journal of Experimental Medicine 205(3):725-735 (2008). PubMed |
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VanDemark A.P., Xin H., McCullough L., Rawlins R., Bentley S., Heroux A., Stillman D.J., Hill C.P. and Formosa T. Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunits. Journal of Biological Chemistry 283(8):5058-5068 (2008). PubMed |
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Fitzpatrick P.F., Bozinovski D.M., Heroux A., Shaw P.G., Valley M.P. and Orville A.M. Mechanistic and structural analyses of the roles of Arg409 and Asp402 in the reaction of the flavoprotein nitroalkane oxidase. Biochemistry. 46(48): 13800-13808 (2007). PubMed |
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Renner M.W., Bochot C., Heroux A., Mansury D. and Battioni P. Structural, electrochemical and spectroscopic properties of a class of dodecasubstituted iron porphyrins bearing four positive charges close to metal. Eur J Inorg Chem. 2426-2433 (2007). |
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VanDemark A.P., Kasten M.M., Ferris E., Heroux A., Hill C.P. and Cairns B.R. Autoregulation of the rsc4 tandem bromodomain by Gcn5 acetylation. Mol Cell. 27(5): 817-828 (2007). PubMed |
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Vandemark A.P., Xin H., McCullough L., Rawlins R., Bentley S., Heroux A., Stillman D.J., Hill C.P.
and Formosa T. Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunits. J Biol Chem. 283(8): 5058-5068 (2007). PubMed |
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Welch B.D., Vandemark A.P., Heroux A., Hill C.P. and Kay M.S. Potent D-peptide inhibitors of HIV-1 entry. Proc Natl Acad Sci USA. 104(43): 16828-16833 (2007). PubMed |
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Zhang L., Shen J., Guarnieri M.T., Heroux A., Yang K. and Zhao R. Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutants. Protein Sci. 16(6): 1024-31 (2007). PubMed |
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Gallagher D.T., Smith N.N., Kim S.K., Heroux A., Robinson H. and Reddy P.T. Structure of the class IV adenylyl cyclase reveals a novel fold. J Mol Biol. 362: 114-122 (2006). PubMed PDB file: 2FJT Jmol viewer |
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Robinson H., Soares A.S., Becker M., Sweet R. and Heroux A. Mail-in crystallography program at Brookhaven National Laboratory's National Synchrotron Light Source. Acta Cryst. D62(11): 1336-1339 (2006). PubMed Full Text |
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Skinner J.M., Cowan M., Buono R., Nolan W., Bosshard H., Robinson H.H., Heroux A., Soares A.S., Schneider D.K. and Sweet R.M. Integrated software for macromolecular crystallography synchrotron beamlines II: revision, robots and a database Acta Cryst. D62(11): 1340-1347 (2006). PubMed Full Text |
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Reyes-Turcu F.E., Horton J.R., Mullally J.E., Heroux A., Cheng X., and Wilkinson K.D. The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitin. Cell. 124(6): 1197-1208 (2006). PubMed PDB files: 2G43, 2G45 Jmol viewer |
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VanDemark A.P., Blanksma M., Ferris E., Heroux A., Hill C.P. and Formosa T. The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition. Mol Cell. 22: 363-374 (2006). PubMed PDB files: 2GCL, 2GCJ Jmol viewer |
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Auperin T.C., Bolduc G.R., Baron M.J., Héroux A., Filman D.J., Madoff L.C., Hogle J.M. Crystal structure of the N-terminal domain of the group B Streptococcus alpha C protein. J Biol Chem. 280(18): 18245-18252 (2005). PubMed Full Text PDB file: 1YWM Jmol viewer |
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Davison P.A., Schubert H.L., Reid J.D., Iorg C.D., Héroux A., Hill C.P. and Hunter C.N. Structural and biochemical characterization of Gun4 suggests a mechanism for its role in chlorophyll biosynthesis. Biochemistry 44(21): 7603-7612 (2005). PubMed PDB files: 1Z3X, 1Z3Y Jmol viewer |
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Loftin I.R., Franke S., Roberts S.A., Weichsel A., Héroux A., Montfort W.R., Rensing C., McEvoy M.M. A novel copper-binding fold for the periplasmic copper resistance protein CusF. Biochemistry. 44(31): 10533-10540 (2005). PubMed PDB file: 1ZEQ Jmol viewer |
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Gopalan G., He Z., Balmer Y., Romano P., Gupta R., Héroux A., Buchanan B.B., Swaminathan K. and Luan S. Structural analysis uncovers a role for redox in regulating FKBP13, an immunophilin of the chloroplast thylakoid lumen. Proc Natl Acad Sci USA. 101(38): 13945-13950 (2004). PubMed Full Text PDB file: 1U79 Jmol viewer |
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Smith N., Mayhew M., Holden M.J., Kelly H., Robinson H., Héroux A., Vilker V.L. and Gallagher D.T. Structure of C73G putidaredoxin from Pseudomonas putida. Acta Cryst. D60(5): 816-822 (2004). PubMed |
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Smith N., Mayhew M., Robinson H., Héroux A., Charlton D., Holden M.J. and Gallagher D.T. Crystallization and phasing of alanine dehydrogenase from Archaeoglobus fulgidus. Acta Cryst. D59(12): 2328-2331 (2003). PubMed |
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Soares A.S., Caspar D.L., Weckert E., Héroux A., Hölzer K., Schroer K., Zellner J., Schneider D., Nolan W. and Sweet R.M. Three-beam interference is a sensitive measure of the efficacy of macromolecular refinement techniques Acta Cryst. D59(10): 1716-1724 (2003). PubMed Full Text |
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Klon E., Héroux A., Ross L.J., Pathak V., Johnson C.A., Piper R.J. and Borhani D.W. Atomic Resolution Structures of Human Dihydrofolate Reductase Complexed with NADPH and Two Lipophilic Antifolates at 1.09 A and 1.05 A Resolution. J Mol Biol. 320(3): 677-693 (2002). PubMed PDB files 1KMS, 1KMV Jmol viewer |
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Héroux A., White E.L., Ross L.J., Kuzin A.P. and Borhani D.W. Substrate Deformation in a Hypoxanthine-Guanine Phosphoribosyltransferase Ternary Complex: The Structural Basis for Catalysis. Structure Fold Des. 8(12): 1309-1318 (2000). PubMed PDB file 1FSG Jmol viewer |
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Héroux A., White E.L., Ross L.J., and Borhani D.W. Crystal Structures of the Toxoplasma gondii Hypoxanthine-Guanine Phosphoribosyltransferase-GMP and IMP Complexes: Comparison of Purine Binding Interactions with the XMP Complex Biochemistry. 38(44): 14485-14494 (1999). PubMed PDB files 1QK4, 1QK3 Jmol viewer |
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Héroux A., White E.L., Ross L.J., Davis R.L. and Borhani D.W. Crystal Structure of Toxoplasma gondii Hypoxanthine-Guanine Phosphoribosyltransferase with XMP, Pyrophosphate and Two Mg2+ Ions Bound: Insights Into the Catalytic Mechanism. Biochemistry. 38(44): 14495-14506 (1999). PubMed PDB file 1QK5 Jmol viewer |
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D.W. Borhani, Héroux A., and Piper J.R. Ultra-high Resolution Crystal Structure of Human Dihydrofolate Reductase Bound to a Lipophilic Antifolate Stanford Synchrotron Radiation Laboratory, 1997 Annual Report. |
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A. Héroux and Brisse F. Acta Cryst. C53 1318-20 (1997). |
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A. Héroux and Brisse F. Acta Cryst. C52 224-26 (1996). |
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M. Belletête, Brisse F., Durocher G., Gravel D., Héroux A. and Popowyck A. J Mol Structure. 297 63-80 (1993). |
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M. Lachapelle, Belletête M., Poulin M., Godbout N., LeGrand F., Héroux A., Brisse F. and Durocher G. J Phys Chem. 95 9764-72 (1991). |
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| Updated June 12, 2008 |
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